EVALUATION OF POLYPEPTIDE
COMPLEXES FROM MARINE SOURCES WITH RELEVANCE IN INITIATION AND PROPAGATION
MECHANISMS OF OSTEOARTICULAR DYSFUNCTIONS
Manuela Diana ENE1, Laura
OLARIU2,*, Brandusa Georgiana DUMITRIU3,
Mihai Alexandru FLOREA4, Raluca
PAPACOCEA5, Natalia ROSOIU6
1 PhD, Eng.
Biochim., Senior Researcher III, Biotehnos
SA, Otopeni, Ilfov, Romania, (diana.ene@biotehnos.com).
2 PhD,
biofiz., Senior Researcher I, Biotehnos
SA, Otopeni, Ilfov, Romania, Coresspondent Member of the Academy of Romanian Scientists (lolariu@biotehnos.com ).
3 PhD, biochim., Senior
Researcher III, Biotehnos SA, Otopeni,
Ilfov, Romania, (dbandrusa@biotehnos.com).
4 PhD, biochim.,
Researcher, Biotehnos SA, Otopeni,
Ilfov, Romania, (alexandru.florea@biotehnos.com).
5 Conf. Univ. Dr. University of
Medicine and Pharmacy "Carol Davila" Bucharest, Romania,
6 Prof.
PhD. Emeritus, Senior Researcher I, PhD, Faculty of Medicine and Doctoral
School of Applied Sciences, Biology / Biochemistry
Section, Ovidius University of
Constanta, Romania, Full member of the Academy of
Romanian Scientists, Biological
Sciences Section President, Bucharest, Romania. (natalia_rosoiu@yahoo.com)
* Corresponding author: Laura Olariu, lolariu@biotehnos.com
Abstract. The aim of the
present study was to obtain a protein fraction rich in enzymes with proteolytic
activity such as serine proteases, chymotrypsins and cysteine proteases and to
investigate preliminary pharmacological effects with therapeutic potential in
osteoarticular diseases. The processing of marine raw material by applying
established and optimized sequences of operations (selective precipitation
followed by fractionation by FPLC) led to well-defined protein fractions rich
in proteolytic enzymes. To evaluate the biological effect of the isolated
polypeptide fractions, an in vitro model on normal human osteoblast (HOB) and
osteoarthritic (HOB-OA) cell lines was carried out in which the modulation of
cell proliferation under the action of the polypeptide complexes was followed.
Key words:
osteoarthritis, trypsin proteinase, HOB-OA, proteases.
DOI 10.56082/annalsarscibio.2023.2.38
